4W6N
Crystal Structure of Full-Length Split GFP Mutant D117C Disulfide Dimer, C 1 2 1 Space Group
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-10-31 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9537 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 181.210, 102.680, 84.130 |
| Unit cell angles | 90.00, 101.44, 90.00 |
Refinement procedure
| Resolution | 88.890 - 3.375 |
| R-factor | 0.3209 |
| Rwork | 0.316 |
| R-free | 0.36260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2b3p |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.494 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 88.890 |
| High resolution limit [Å] | 3.375 |
| Number of reflections | 20957 |
| <I/σ(I)> | 6.64 |
| Completeness [%] | 97.9 |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 10% PEG6000, 0.1M Hepes pH 6.5 |
