4W6M
Crystal Structure of Full-Length Split GFP Mutant D117C Disulfide Dimer, P 63 Space Group
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-08-09 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 63 |
| Unit cell lengths | 170.500, 170.500, 79.570 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 73.830 - 2.794 |
| R-factor | 0.263 |
| Rwork | 0.261 |
| R-free | 0.28500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2b3p |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.218 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE: DEV_1555)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 73.830 |
| High resolution limit [Å] | 2.794 |
| Number of reflections | 32658 |
| <I/σ(I)> | 4.81 |
| Completeness [%] | 99.2 |
| Redundancy | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | 10% PEG3350, 0.1M NaAcetate pH 4.6, 0.2M NaCl |
