4W6L

Crystal Structure of Full-Length Split GFP Mutant D117C Disulfide Dimer, I 41 2 2 Space Group

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-08-09
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.0717
Spacegroup name	I 41 2 2
Unit cell lengths	108.430, 108.430, 101.470
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	76.672 - 2.450
R-factor	0.2548
Rwork	0.252
R-free	0.27800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2b3p
RMSD bond length	0.010
RMSD bond angle	1.233
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall
Low resolution limit [Å]	76.670
High resolution limit [Å]	2.450
Number of reflections	11419
<I/σ(I)>	23.95
Completeness [%]	99.8
Redundancy	13

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	298	1.5M NaNO3, 0.1M NaAcetate pH 5.0