4W6J
Crystal Structure of Full-Length Split GFP Mutant D117C Disulfide Dimer, P 31 2 1 Space Group
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-08-09 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 113.740, 113.740, 82.460 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 98.500 - 1.702 |
| R-factor | 0.191 |
| Rwork | 0.189 |
| R-free | 0.21200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2b3p |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.178 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE: DEV_1555)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 98.500 |
| High resolution limit [Å] | 1.700 |
| Number of reflections | 67258 |
| <I/σ(I)> | 16.58 |
| Completeness [%] | 99.5 |
| Redundancy | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 298 | 35% MPD, 0.1M Na Acetate pH 4.5 |
