4W6I

Crystal Structure of Full-Length Split GFP Mutant D190C Disulfide Dimer, P 21 21 21 Space Group

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-08-09
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.0717
Spacegroup name	P 21 21 21
Unit cell lengths	57.350, 67.600, 150.580
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	53.390 - 2.625
R-factor	0.273
Rwork	0.268
R-free	0.31600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2b3p
RMSD bond length	0.009
RMSD bond angle	1.160
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE: DEV_1555))

Data quality characteristics

	Overall
Low resolution limit [Å]	53.390
High resolution limit [Å]	2.625
Number of reflections	17334
<I/σ(I)>	5.91
Completeness [%]	95.4
Redundancy	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	298	1.4M MgSO4, 0.1M BTP pH 7.4