4W5X

The structure of Vaccina virus H7 protein displays A Novel Phosphoinositide binding fold required for membrane biogenesis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2012-08-11
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	C 1 2 1
Unit cell lengths	112.785, 34.207, 67.200
Unit cell angles	90.00, 99.57, 90.00

Refinement procedure

Resolution	39.485 - 2.002
R-factor	0.2007
Rwork	0.196
R-free	0.24710
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	0.957
Refinement software	PHENIX ((phenix.refine: 1.6_289))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.070
High resolution limit [Å]	2.000	2.000
Number of reflections	16966
<I/σ(I)>	17
Completeness [%]	97.6	87.5
Redundancy	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	8.8	293	0.08M Bis-Tris phosphate/citric acid, 18% (w/v) PEG 3350