4W5T
The Crystal Structure of Human Argonaute2 Bound to a Guide and Target RNA Containing Seed Pairing from 2-7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-01-24 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97950 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.636, 116.468, 69.822 |
| Unit cell angles | 90.00, 92.33, 90.00 |
Refinement procedure
| Resolution | 35.291 - 2.500 |
| R-factor | 0.1748 |
| Rwork | 0.173 |
| R-free | 0.21510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4w5o |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.194 |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.820 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.074 | 0.398 |
| Number of reflections | 29434 | |
| <I/σ(I)> | 12.5 | 3.3 |
| Completeness [%] | 95.8 | 97.6 |
| Redundancy | 3.5 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | PEG 3350, Isopropanol, Phenol, Tris, Magnesium |
