4W4Q
Glucose isomerase structure determined by serial femtosecond crystallography at SACLA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | FREE ELECTRON LASER |
| Source details | SACLA BEAMLINE BL3 |
| Synchrotron site | SACLA |
| Beamline | BL3 |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2013-10-13 |
| Detector | MPCCD |
| Wavelength(s) | 1.23 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 94.000, 100.000, 103.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.980 - 2.000 |
| R-factor | 0.16094 |
| Rwork | 0.159 |
| R-free | 0.19580 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.713 |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 2.000 |
| Number of reflections | 33122 |
| <I/σ(I)> | 3.6 |
| Completeness [%] | 99.9 |
| Redundancy | 85 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 8.5 | 278 | 40%(w/v) PEG4000, 0.2 M lithium sulfate, 0.1 M Tris-HCl |
