4W4I
Crystal structure of EspG3 from the ESX-3 type VII secretion system of M. tuberculosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-09-19 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.116 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 74.980, 96.310, 94.450 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.155 - 2.850 |
| R-factor | 0.2296 |
| Rwork | 0.225 |
| R-free | 0.27440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4w4j |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.661 |
| Data reduction software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: dev_1727)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.920 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Rmerge | 1.560 | |
| Number of reflections | 8298 | |
| <I/σ(I)> | 10.8 | 1.1 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.1 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M magnesium sulfate, 20% (w/v) polyethylene glycol [PEG] 3350 |
