4W4G
Postcleavage state of 70S bound to HigB toxin and AAA (lysine) codon
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-11-30 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97920 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 214.112, 453.875, 607.594 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.962 - 3.300 |
| R-factor | 0.2108 |
| Rwork | 0.209 |
| R-free | 0.23840 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.834 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | PHENIX ((phenix.refine: 1.8.3_1479)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 35.000 | 3.400 | |
| High resolution limit [Å] | 3.300 | 20.000 | 3.300 |
| Rmerge | 0.170 | 0.027 | 0.772 |
| Rmeas | 0.194 | 0.031 | 0.886 |
| Total number of observations | 3393142 | ||
| Number of reflections | 827408 | 3039 | 70329 |
| <I/σ(I)> | 8.9 | 42.9 | 1.81 |
| Completeness [%] | 94.3 | 69.8 | 94.1 |
| Redundancy | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 4% PEG 20k, 4% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN |
