4W2I
Crystal structure of the Thermus thermophilus 70S ribosome in complex with negamycin, mRNA and three deacylated tRNAs in the A, P and E sites
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-12-09 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.97900 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 208.250, 443.610, 619.300 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 124.249 - 2.700 |
R-factor | 0.2122 |
Rwork | 0.210 |
R-free | 0.25670 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.026 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 360.635 | 2.770 | |
High resolution limit [Å] | 2.700 | 12.070 | 2.700 |
Rmerge | 0.128 | 0.013 | 0.013 |
Rmeas | 0.027 | 1.481 | |
Number of reflections | 1505170 | 16611 | 112443 |
<I/σ(I)> | 8.77 | 31.74 | 0.99 |
Completeness [%] | 97.2 | 90.6 | 98.6 |
Redundancy | 3.504 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.6 | 292 | 0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME, VAPOR DIFFUSION, temperature 292K |