4W1Y
Crystal structure of Escherichia coli Tryptophanase in 'semi-holo' form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-08-01 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 109.971, 109.971, 238.403 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 3.200 |
| R-factor | 0.21772 |
| Rwork | 0.214 |
| R-free | 0.28972 |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.385 |
| Refinement software | REFMAC (5.4.0077) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 3.300 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.519 | |
| Number of reflections | 22389 | |
| <I/σ(I)> | 19.2 | 3.1 |
| Completeness [%] | 94.8 | 97.2 |
| Redundancy | 5.9 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 13-16 % AMMONIUM SULFATE, 1-5 MM PLP, 50 MM TRIS |
