4V9M

70S Ribosome translocation intermediate FA-4.2A containing elongation factor EFG/FUSIDIC ACID/GDP, mRNA, and tRNA bound in the pe*/E state.

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2012-06-07
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.03322
Spacegroup name	C 1 2 1
Unit cell lengths	307.210, 670.437, 350.402
Unit cell angles	90.00, 92.37, 90.00

Refinement procedure

Resolution	40.000 - 4.000
R-factor	0.2813
Rwork	0.281
R-free	0.32910
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 3F1E 3F1F 2WRI 2WRJ
RMSD bond length	0.019
RMSD bond angle	1.068
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	CNS (1.2)

Data quality characteristics

	Overall
Low resolution limit [Å]	350.102
High resolution limit [Å]	3.983
Number of reflections	2518956

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM
1				2.9% PEG 20K, 2.8% PPG P400, 5.1% MME 550, KSCN 200 mM, TRIS ACETATE 100mM