Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-08-19 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 211.594, 455.434, 616.940 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.790 - 3.350 |
| R-factor | 0.223 |
| Rwork | 0.223 |
| R-free | 0.26100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 3KIQ 3KIR |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.200 |
| Data reduction software | CNS |
| Data scaling software | CNS |
| Phasing software | CNS |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.400 |
| High resolution limit [Å] | 3.350 | 3.350 |
| Rmerge | 0.150 | 1.060 |
| Number of reflections | 842970 | |
| <I/σ(I)> | 9.3 | 1.6 |
| Completeness [%] | 99.7 | 99.8 |
| Redundancy | 5.1 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.1 | 0.1 M TRIS-HAC PH 7.2, 0.2 M KSCN, 4.1%-4.3% (W/V) PEG 20K AND 4.1%-4.3% (W/V) PEG 550MME |
