4V8K
Crystal structure of the LH1-RC complex from Thermochromatium tepidum in P21 form
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-01-30 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 167.159, 145.429, 210.528 |
| Unit cell angles | 90.00, 108.50, 90.00 |
Refinement procedure
| Resolution | 43.794 - 3.006 |
| R-factor | 0.3364 |
| Rwork | 0.335 |
| R-free | 0.35640 |
| Structure solution method | MIR |
| RMSD bond length | 0.029 |
| RMSD bond angle | 2.312 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 167629 | |
| <I/σ(I)> | 9.14 | 1.73 |
| Completeness [%] | 88.5 | 71.8 |
| Redundancy | 5.4 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 293 | 20mM Tris-HCl (pH 7.4), 50mM CaCl2, 0.54% DPC, 2% glycerol, 14% PEG 3000 , VAPOR DIFFUSION, SITTING DROP, temperature 293K |
