4V7V

Crystal structure of the E. coli ribosome bound to clindamycin.

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 315
Wavelength(s)	1.11589
Spacegroup name	P 21 21 21
Unit cell lengths	211.080, 434.461, 618.916
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	76.348 - 3.289
R-factor	0.1897
Rwork	0.189
R-free	0.24100
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.382
Data scaling software	SCALEPACK
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	80.000	80.000	3.380
High resolution limit [Å]	3.289	8.140	3.289
Rmerge	0.120	0.049	0.743
Number of reflections	700416
<I/σ(I)>	10.3
Completeness [%]	83.2	93.5	70.6
Redundancy	6.3	10.9	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH		291	4.3% PEG 8000, 4.9% MPD, 10 mM Tris, 20 mM MES, pH 6.5-7.0, 3.8 mM MgCl2, 380 mM NH4Cl, 5.5 mM putrescine, 5 mM spermidine, 0.25 mM EDTA, microbatch, temperature 291K