4V7J
Structure of RelE nuclease bound to the 70S ribosome (precleavage state)
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2009-06-12 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 211.571, 451.963, 622.438 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.300 |
| R-factor | 0.219 |
| Rwork | 0.219 |
| R-free | 0.24700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2WH1 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.170 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.400 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.016 | 0.012 |
| Number of reflections | 883810 | |
| <I/σ(I)> | 9.8 | 1.95 |
| Completeness [%] | 99.8 | 99.6 |
| Redundancy | 8.46 | 7.43 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 293 | 0.1M Tris-HAc, 0.2M KSCN, 3-4.5% w/v PEG 20k, 3-4.5% PEG 550 monomethylether, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Tris-HAc | ||
| 2 | 1 | 1 | KSCN | ||
| 3 | 1 | 1 | PEG 20k | ||
| 4 | 1 | 1 | PEG 550 monomethylether | ||
| 5 | 1 | 2 | Tris-HAc | ||
| 6 | 1 | 2 | KSCN | ||
| 7 | 1 | 2 | PEG 20k | ||
| 8 | 1 | 2 | PEG 550 monomethylether |
