4V4C
Crystal Structure of Pyrogallol-Phloroglucinol Transhydroxylase from Pelobacter acidigallici
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-04-30 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.7426 |
| Spacegroup name | P 1 |
| Unit cell lengths | 174.022, 179.641, 181.225 |
| Unit cell angles | 63.69, 63.98, 64.90 |
Refinement procedure
| Resolution | 24.980 - 2.350 |
| R-factor | 0.198 |
| Rwork | 0.198 |
| R-free | 0.25400 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 2.800 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | SHARP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.630 | 2.390 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.134 | 0.600 |
| Number of reflections | 635960 | |
| <I/σ(I)> | 7 | 1.2 |
| Completeness [%] | 90.1 | 87.2 |
| Redundancy | 2.3 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | PEG 8000, sodium acetate, potassium phosphate, sodium dithionite, sodium cacodylate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 291.0K |
