4V3D
The CIDRa domain from HB3var03 PfEMP1 bound to endothelial protein C receptor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-11-17 |
| Detector | PILATUS |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 66.130, 94.670, 290.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 53.290 - 2.650 |
| R-factor | 0.2241 |
| Rwork | 0.222 |
| R-free | 0.25520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1l8j |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.250 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.290 | 2.790 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.090 | 0.580 |
| Number of reflections | 25427 | |
| <I/σ(I)> | 9.2 | 2.3 |
| Completeness [%] | 95.3 | 95.4 |
| Redundancy | 3.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 0.2 M NANO3, 0.1 M BTP PH 8.5, 20% PEG 3350 |
