4V34
The Structure of A-PGS from Pseudomonas aeruginosa (SeMet derivative)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-03-06 |
| Detector | MARRESEARCH |
| Spacegroup name | P 41 2 2 |
| Unit cell lengths | 93.690, 93.690, 166.160 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.678 - 3.100 |
| R-factor | 0.1832 |
| Rwork | 0.182 |
| R-free | 0.21260 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.637 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.180 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.310 | 1.500 |
| Number of reflections | 25682 | |
| <I/σ(I)> | 10.1 | 2.1 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 12.3 | 12.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.7 | 0.085M MES PH 5.7; 1.53M (NH4)2SO4; 15%(V/V) GLYCEROL; 0.0075M COCL2 |
