4V30
Cereblon isoform 4 from Magnetospirillum gryphiswaldense in complex with Lenalidomide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-11-26 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.682, 59.743, 88.064 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.290 - 1.850 |
| R-factor | 0.18786 |
| Rwork | 0.185 |
| R-free | 0.23875 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.172 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.300 | 1.960 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.070 | 0.420 |
| Number of reflections | 26228 | |
| <I/σ(I)> | 8.41 | 1.96 |
| Completeness [%] | 98.7 | 98 |
| Redundancy | 2.86 | 2.81 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 15 %(W/V) PEG 6000, 5 %(W/V) GLYCEROL |
