4V1J
Structure of Neisseria meningitidis Major Pillin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS |
| Spacegroup name | P 6 |
| Unit cell lengths | 105.060, 105.060, 23.060 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 23.060 - 1.430 |
| R-factor | 0.14023 |
| Rwork | 0.138 |
| R-free | 0.17586 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hi2 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.580 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.060 | 1.470 |
| High resolution limit [Å] | 1.430 | 1.430 |
| Rmerge | 0.050 | 0.600 |
| Number of reflections | 27504 | |
| <I/σ(I)> | 19.3 | 2.5 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 6.8 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.1M SODIUM HEPES PH7.5, 45% V/V PEG 600 |
