4V18
SeMet structure of a novel carbohydrate binding module from glycoside hydrolase family 5 glucanase from Ruminococcus flavefaciens FD-1
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-06-21 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 131.686, 131.686, 104.711 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 77.130 - 2.280 |
| R-factor | 0.18542 |
| Rwork | 0.182 |
| R-free | 0.24743 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.867 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | AutoSol |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 77.130 | 2.350 |
| High resolution limit [Å] | 2.280 | 2.280 |
| Rmerge | 0.060 | 0.540 |
| Number of reflections | 16115 | |
| <I/σ(I)> | 16.4 | 3.2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.2 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 0.3 M AMMONIUM SULFATE, 24% (W/V) POLYETHYLENE GLYCOL 3350. CRYO 30% GLYCEROL ADDED TO ABOVE, pH 7 |
