4V0N
Crystal structure of BBS1N in complex with ARL6DN, soaked with mercury
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 2MF |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 123.745, 123.745, 443.709 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 96.498 - 3.131 |
| R-factor | 0.2145 |
| Rwork | 0.213 |
| R-free | 0.24900 |
| Structure solution method | OTHER |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.476 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.240 |
| High resolution limit [Å] | 3.130 | 3.130 |
| Rmerge | 0.110 | 0.790 |
| Number of reflections | 70112 | |
| <I/σ(I)> | 12.6 | 1.7 |
| Completeness [%] | 99.2 | 91.8 |
| Redundancy | 13.2 | 11.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 29% PEG400, 0.1M TRIS PH 8 |
