4V0M
Crystal structure of BBS1N in complex with ARL6DN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 2MF |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 124.785, 124.785, 441.840 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 87.128 - 3.450 |
| R-factor | 0.2242 |
| Rwork | 0.221 |
| R-free | 0.28130 |
| Structure solution method | OTHER |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.610 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.550 |
| High resolution limit [Å] | 3.450 | 3.450 |
| Rmerge | 0.120 | 0.820 |
| Number of reflections | 51878 | |
| <I/σ(I)> | 9.1 | 1 |
| Completeness [%] | 99.1 | 97.6 |
| Redundancy | 6.5 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 29% PEG400, 0.1M TRIS PH 8 |
