4V0L
Crystal structure of the CrARL6DN in the GTP bound form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 118.721, 118.721, 146.702 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.515 - 2.197 |
| R-factor | 0.1862 |
| Rwork | 0.184 |
| R-free | 0.21890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2h57 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.189 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.030 | 0.550 |
| Number of reflections | 29942 | |
| <I/σ(I)> | 21.1 | 1.5 |
| Completeness [%] | 99.9 | 99.1 |
| Redundancy | 12.7 | 12.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 30% V/V PENTAERYTHRITOL ETHOXYLATE, 50MM BIS-TRIS PH 6.5, 50MM AMMONIUM SULFATE |
