4V06
Crystal structure of human tryptophan hydroxylase 2 (TPH2), catalytic domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-07-12 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 95.960, 100.199, 89.009 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.504 - 2.630 |
| R-factor | 0.2001 |
| Rwork | 0.198 |
| R-free | 0.24240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2pah |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.531 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.500 | 2.750 |
| High resolution limit [Å] | 2.630 | 2.630 |
| Rmerge | 0.120 | 1.320 |
| Number of reflections | 26141 | |
| <I/σ(I)> | 11.9 | 2.5 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 5.6 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.20M SODIUM ACETATE; 0.1M BIS-TRIS- PROPANE PH 6.5; 20.0% PEG 3350; 10.0% ETHYLENE GLYCOL |
