4V02

MinC:MinD cell division protein complex, Aquifex aeolicus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	P 65 2 2
Unit cell lengths	130.618, 130.618, 298.507
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.171 - 2.700
R-factor	0.1907
Rwork	0.188
R-free	0.23570
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.016
RMSD bond angle	1.630
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.850
High resolution limit [Å]	2.700	2.700
Rmerge	0.100	0.630
Number of reflections	42285
<I/σ(I)>	20.6	4.6
Completeness [%]	100.0	100
Redundancy	14.3	12.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1