4V00
Structural and functional characterization of a novel monotreme- specific protein from the milk of the platypus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-10-08 |
Detector | ADSC CCD |
Spacegroup name | P 1 |
Unit cell lengths | 57.152, 59.721, 63.074 |
Unit cell angles | 80.15, 82.98, 89.27 |
Refinement procedure
Resolution | 61.680 - 1.820 |
R-factor | 0.16702 |
Rwork | 0.166 |
R-free | 0.18786 |
Structure solution method | SAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.009 |
RMSD bond angle | 1.162 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | Auto-Rickshaw |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.750 | 1.860 |
High resolution limit [Å] | 1.820 | 1.820 |
Rmerge | 0.090 | 0.810 |
Number of reflections | 72773 | |
<I/σ(I)> | 15.1 | 2.2 |
Completeness [%] | 99.5 | 91.2 |
Redundancy | 7.4 | 6.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.6 | 281 | THE PROTEIN WAS AT 4 MG/ML IN TRIS BUFFERED SALINE; THE RESERVOIR WAS 68 MM AMMONIUM ACETATE, 25.1% W/V PEG 8K, 100 MM SODIUM CACODYLATE BUFFER AT PH 6.6; DONE AT 8C. |