4UZY
Crystal structure of the Chlamydomonas IFT70 and IFT52 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-09-21 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 63 |
| Unit cell lengths | 143.177, 143.177, 88.693 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 71.588 - 2.477 |
| R-factor | 0.1878 |
| Rwork | 0.185 |
| R-free | 0.23040 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.324 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.000 | 2.650 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.100 | 1.500 |
| Number of reflections | 71955 | |
| <I/σ(I)> | 17.7 | 1.3 |
| Completeness [%] | 99.5 | 97.2 |
| Redundancy | 10.5 | 9.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 20% TACSIMATE PH 7.0 |
