4UZS
Crystal structure of Bifidobacterium bifidum beta-galactosidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-06-06 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 95.701, 99.241, 110.757 |
| Unit cell angles | 90.00, 106.47, 90.00 |
Refinement procedure
| Resolution | 35.772 - 1.740 |
| R-factor | 0.1555 |
| Rwork | 0.153 |
| R-free | 0.19280 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.165 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | autoSHARP |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.700 | 1.800 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.090 | 0.650 |
| Number of reflections | 200160 | |
| <I/σ(I)> | 11.8 | 1.8 |
| Completeness [%] | 98.5 | 89.9 |
| Redundancy | 3.1 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 292 | 20% (W/V) POLYETHYLENE GLYCOL 3, 350, 0.2 M DIBASIC AMMONIUM TARTRATE AND 4% (W/V) 1-PROPANOL AT 292 K, pH 6 |
