4UZ1

STRUCTURE OF THE WNT DEACYLASE NOTUM - CRYSTAL FORM III - 1.4A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-12-07
Detector	DECTRIS PIXEL
Spacegroup name	P 21 21 21
Unit cell lengths	59.969, 69.843, 77.535
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	51.890 - 1.400
R-factor	0.14447
Rwork	0.144
R-free	0.18045
Structure solution method	OTHER
Starting model (for MR)	NONE
RMSD bond length	0.013
RMSD bond angle	1.567
Data reduction software	XDS
Data scaling software	Aimless
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall
Low resolution limit [Å]	77.000
High resolution limit [Å]	1.400
Rmerge	0.050
Number of reflections	63440
<I/σ(I)>	14.1
Completeness [%]	98.0
Redundancy	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		4.6		1.5 M AMMONIUM SULPHATE, 0.1 M SODIUM ACETATE PH 4.6