4UYP
High resolution structure of the third cohesin ScaC in complex with the ScaB dockerin with a mutation in the N-terminal helix (IN to SI) from Acetivibrio cellulolyticus displaying a type I interaction.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-05-05 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 107.750, 107.750, 100.810 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 76.190 - 1.490 |
| R-factor | 0.15696 |
| Rwork | 0.155 |
| R-free | 0.18536 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ccl |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.867 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | BALBES |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 76.190 | 1.530 |
| High resolution limit [Å] | 1.490 | 1.490 |
| Rmerge | 0.070 | 0.590 |
| Number of reflections | 96788 | |
| <I/σ(I)> | 15.9 | 2.7 |
| Completeness [%] | 99.9 | 99 |
| Redundancy | 6.6 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.5 M AMMONIUM SULFATE, 0.1 M HEPES PH 7.5, 30%(V/V) MPD |
