4UY3
Cytoplasmic domain of bacterial cell division protein ezra
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-07-10 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 96.135, 96.135, 97.128 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.560 - 2.600 |
| R-factor | 0.21112 |
| Rwork | 0.209 |
| R-free | 0.24894 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | TO BE DEPOSITED |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.317 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.560 | 2.720 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.080 | 0.970 |
| Number of reflections | 14588 | |
| <I/σ(I)> | 15.3 | 2.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 14.4 | 14.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.1M TRIS PH 7.5, 34% MPD |
