4UXV
Cytoplasmic domain of bacterial cell division protein EzrA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-10-16 |
| Detector | DECTRIS PILATUS 2M |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 222.703, 222.703, 184.115 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.044 - 3.961 |
| R-factor | 0.3199 |
| Rwork | 0.317 |
| R-free | 0.34830 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.785 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.170 | 4.160 |
| High resolution limit [Å] | 3.950 | 3.950 |
| Rmerge | 0.100 | 0.350 |
| Number of reflections | 15330 | |
| <I/σ(I)> | 20.8 | 3.5 |
| Completeness [%] | 99.6 | 99.2 |
| Redundancy | 22.4 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.7 | 50 MM MES PH 6.7, 1 M SODIUM CITRATE, 0.2M NDSB-201, 0.1M AMMONIUM SULPHATE |
