4UWX
Structure of liprin-alpha3 in complex with mDia1 Diaphanous- inhibitory domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-12-20 |
| Detector | DECTRIS PILATUS 2M |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 121.090, 49.381, 106.370 |
| Unit cell angles | 90.00, 97.86, 90.00 |
Refinement procedure
| Resolution | 32.317 - 1.650 |
| R-factor | 0.177 |
| Rwork | 0.175 |
| R-free | 0.21010 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1z2c |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.005 |
| Data reduction software | MOSFLM |
| Data scaling software | TRUNCATE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 105.370 | 1.660 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.070 | 0.730 |
| Number of reflections | 75030 | |
| <I/σ(I)> | 9.37 | 1.33 |
| Completeness [%] | 99.7 | 99.9 |
| Redundancy | 3.13 | 3.24 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 0.2 M NACL, 0.1 M TRIS/HCL PH 8.0, 20% PEG6000 |
