4UVQ

PatG Domain of Unknown Function

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	93
Detector technology	PIXEL
Collection date	2012-12-15
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 21 21 2
Unit cell lengths	64.110, 95.190, 40.160
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	53.175 - 1.724
R-factor	0.1764
Rwork	0.175
R-free	0.19950
Structure solution method	SAD
Starting model (for MR)	NONE
RMSD bond length	0.009
RMSD bond angle	0.932
Data reduction software	XDS
Data scaling software	SCALEPACK
Phasing software	PHENIX (AUTOSOL)
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	53.170	1.770
High resolution limit [Å]	1.720	1.720
Rmerge	0.140	0.900
Number of reflections	26646
<I/σ(I)>	12.1	3.4
Completeness [%]	99.9	100
Redundancy	14	11.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				0.04 M POTASSIUM PHOSPHATE, 16 % (W/V) PEG 8000, AND 20 % (V/V) GLYCEROL