4UVM
In meso crystal structure of the POT family transporter PepTSo
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-02-23 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 86.830, 86.830, 219.820 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 59.135 - 3.000 |
| R-factor | 0.2222 |
| Rwork | 0.220 |
| R-free | 0.25800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xut |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.771 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.130 | 3.080 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.080 | 0.830 |
| Number of reflections | 17156 | |
| <I/σ(I)> | 13 | 1.9 |
| Completeness [%] | 97.5 | 98.2 |
| Redundancy | 4.9 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 4.5 | 20-23 %(V/V) PEG 400, 0.1 M SODIUM ACETATE PH 4.5-4.8, 0.08-0.12 M SODIUM CITRATE. CRYSTALS WERE GROWN BY THE LCP METHOD USING 7.8 MAG AS THE HOSTING LIPID. |
