4UVJ
Cohesin subunit Scc3 from yeast, 674-1072
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 56.787, 58.022, 80.179 |
| Unit cell angles | 80.68, 82.00, 63.99 |
Refinement procedure
| Resolution | 50.000 - 2.100 |
| R-factor | 0.1893 |
| Rwork | 0.186 |
| R-free | 0.24678 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.872 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.120 | 0.410 |
| Number of reflections | 48926 | |
| <I/σ(I)> | 5 | 1.8 |
| Completeness [%] | 97.5 | 97.5 |
| Redundancy | 1.9 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
