4UV2
Structure of the curli transport lipoprotein CsgG in a non-lipidated, pre-pore conformation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-05-05 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 101.330, 103.600, 141.740 |
| Unit cell angles | 111.33, 90.55, 118.21 |
Refinement procedure
| Resolution | 29.765 - 2.800 |
| R-factor | 0.1903 |
| Rwork | 0.188 |
| R-free | 0.23370 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.314 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | XSCALE |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.150 | 0.810 |
| Number of reflections | 112419 | |
| <I/σ(I)> | 9.82 | 2 |
| Completeness [%] | 98.7 | 98.3 |
| Redundancy | 11.2 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.2 | 100 MM SODIUM ACETATE PH 4.2, 8% PEG 4000, 100 MM SODIUM MALONATE PH 7.0 |
