4UUT
Crystal structure of the Ultrabithorax protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-10-21 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 41 3 2 |
| Unit cell lengths | 98.648, 98.648, 98.648 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 69.750 - 2.800 |
| R-factor | 0.2192 |
| Rwork | 0.217 |
| R-free | 0.23660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | LOCAL STRUCTURE OF UBX |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.030 |
| Data reduction software | autoPROC |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 69.750 | 3.130 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.060 | 0.580 |
| Number of reflections | 4428 | |
| <I/σ(I)> | 25.8 | 3.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8.7 | 8.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 20% 1-4 BUTANEDIOL, 0.2M LISO4, 0.1M MES PH 6 |
