4UUN

Trichomonas vaginalis lactate dehydrogenase in complex with NADH

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Temperature [K]	100
Detector technology	CCD
Collection date	2011-09-22
Detector	OXFORD
Spacegroup name	P 1 21 1
Unit cell lengths	82.570, 59.610, 86.490
Unit cell angles	90.00, 116.18, 90.00

Refinement procedure

Resolution	45.829 - 1.780
Rwork	0.154
R-free	0.19040
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	HOMOLOGY MODEL BASED ON PDB ENTRY 5MDH
RMSD bond length	0.005
RMSD bond angle	1.063
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.300	1.830
High resolution limit [Å]	1.780	1.780
Rmerge	0.071	0.751
Number of reflections	67400
<I/σ(I)>	13.18	1.17
Completeness [%]	89.9	37.5
Redundancy	3.23

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.8		20 MG/ML PROTEIN, 100 MM HEPES PH 6.8, 8% PEG-6000, 5% MPD