4UUC
Crystal structure of human ASB11 ankyrin repeat domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-07-06 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 43.215, 64.375, 89.603 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 52.281 - 1.800 |
| R-factor | 0.1635 |
| Rwork | 0.162 |
| R-free | 0.20230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zkj |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.752 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 89.600 | 1.840 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.100 | 0.730 |
| Number of reflections | 23795 | |
| <I/σ(I)> | 11.1 | 2.3 |
| Completeness [%] | 99.7 | 98.7 |
| Redundancy | 5.9 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 0.2M AMMONIUM ACETATE, 25% PEG3350, 0.1M BIS-TRIS PH 5.5 |
