4UUA
Crystal structure of zebrafish Sirtuin 5 in complex with 3S-Z-amino- succinylated CPS1-peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-04-17 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 87.190, 87.190, 313.870 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.870 - 2.800 |
| R-factor | 0.21071 |
| Rwork | 0.208 |
| R-free | 0.26138 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2nyr |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.690 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.250 | |
| Number of reflections | 18292 | |
| <I/σ(I)> | 10.6 | 1.3 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 10.5 | 11.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.6 | 20% PEG3350, 0.1 M HEPES PH 7.6 |
