4UU2
Ferulic acid decarboxylase from Enterobacter sp., single mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-12-13 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 39.210, 83.880, 132.020 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.940 - 1.490 |
| R-factor | 0.1592 |
| Rwork | 0.158 |
| R-free | 0.17650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | NATIVE STRUCTURE OF THE SAME PROTEIN |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.030 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.900 | 1.590 |
| High resolution limit [Å] | 1.490 | 1.490 |
| Rmerge | 0.100 | 0.670 |
| Number of reflections | 71286 | |
| <I/σ(I)> | 11.8 | 2.4 |
| Completeness [%] | 98.7 | 93.2 |
| Redundancy | 8.4 | 7.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2 M AMMONIUM SULFATE, 0.1 M BIS-TRIS PH 5.5 AND 25 % W/V PEG 3350 20MG/ML PROTEIN (IN 100 MM SUCCINIC ACID, SODIUM DIHYDROGEN PHOSPHATE MONOHYDRATE AND GLYCINE, PH 7.0) |
