4UU1
CRYSTAL STRUCTURE OF (SR) CALCIUM-ATPASE E2(TG) IN THE PRESENCE OF DOPC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-02-10 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 71.703, 71.703, 591.318 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 73.915 - 2.800 |
| R-factor | 0.2246 |
| Rwork | 0.221 |
| R-free | 0.29290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2c8k |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.181 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 73.900 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.090 | 1.210 |
| Number of reflections | 39919 | |
| <I/σ(I)> | 17.06 | 2.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13.3 | 13.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.8 | 18 % (V/V) PEG2000, 100 MM MGSO4, 3 % (V/V) TERT-BUOH, pH 6.8 |
