4UTC
Crystal structure of dengue 2 virus envelope glycoprotein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-09-27 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 105.462, 105.462, 165.895 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.080 |
| R-factor | 0.214 |
| Rwork | 0.211 |
| R-free | 0.27880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1oan |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.330 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.260 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.100 | 0.610 |
| Number of reflections | 17615 | |
| <I/σ(I)> | 23.9 | 2.5 |
| Completeness [%] | 99.3 | 96 |
| Redundancy | 17.1 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 100MM TRIS PH 8.0 86 MM NAFORMATE 16% PEG 3,350 |
