4UT2
X-ray structure of the human PP1 gamma catalytic subunit treated with ascorbate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-02-23 |
| Detector | DECTRIS PILATUS 2M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.550, 90.240, 91.130 |
| Unit cell angles | 90.00, 90.05, 90.00 |
Refinement procedure
| Resolution | 64.120 - 1.960 |
| R-factor | 0.16836 |
| Rwork | 0.167 |
| R-free | 0.20065 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2o8a |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.998 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.120 | 2.010 |
| High resolution limit [Å] | 1.960 | 1.960 |
| Rmerge | 0.080 | 0.610 |
| Number of reflections | 42357 | |
| <I/σ(I)> | 11.7 | 2.5 |
| Completeness [%] | 94.7 | 95.6 |
| Redundancy | 4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 9 | 19.3 % PEG 3350, 200 MM NACL, 100 MM TRIS PH 9.0 SOAKING IN RESERVOIR ENRICHED BY 25MM ASCORBATE FOR 30 MINUTES CRYOPROTECTION 20% GLYCEROL |
