4USQ
Structure of flavin-containing monooxygenase from Cellvibrio sp. BR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 120 |
| Detector technology | PIXEL |
| Collection date | 2014-03-12 |
| Detector | DECTRIS PILATUS |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 115.410, 95.090, 92.370 |
| Unit cell angles | 90.00, 126.26, 90.00 |
Refinement procedure
| Resolution | 22.350 - 2.390 |
| R-factor | 0.24783 |
| Rwork | 0.246 |
| R-free | 0.28662 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4a9w |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.662 |
| Data reduction software | xia2 |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.440 | 2.450 |
| High resolution limit [Å] | 2.390 | 2.390 |
| Rmerge | 0.090 | 0.500 |
| Number of reflections | 31344 | |
| <I/σ(I)> | 9 | 2.9 |
| Completeness [%] | 98.1 | 98.4 |
| Redundancy | 3.2 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 1.5 M AMMONIUM SULFATE AND 1% (W/V)PROPAN-2-OL PH 7.0 |
