4USI
Nitrogen regulatory protein PII from Chlamydomonas reinhardtii in complex with MgATP and 2-oxoglutarate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-06-24 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.040, 90.170, 46.180 |
| Unit cell angles | 90.00, 96.47, 90.00 |
Refinement procedure
| Resolution | 37.900 - 1.450 |
| R-factor | 0.187 |
| Rwork | 0.185 |
| R-free | 0.21587 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xzw |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.460 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.900 | 1.540 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.050 | 0.600 |
| Number of reflections | 59345 | |
| <I/σ(I)> | 13.6 | 2.02 |
| Completeness [%] | 97.7 | 93.5 |
| Redundancy | 3.28 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.15 M (NH4)2SO4, 0.1 M HEPES, PH 7.0, 20% (W/V) PEG 4000 |
